EPO admits patent mistake

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Emerging EPO and EPO receptor regulators and signal transducers.

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Autocrine stimulation by erythropoietin (Epo) requires Epo secretion.

Erythropoietin (Epo) autocrine stimulation has been implicated in erythroblastic leukemia. To examine whether this stimulation could occur intracellularly, we developed Epo autocrine models of stimulation in the human pluripotent UT-7 cell line. Retroviral expression of Epo totally abolished the growth factor requirement of UT-7 cells. Autonomous proliferation was not cell density-dependent and...

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Selected anti-Epo receptor antibodies predict Epo receptor expression.

In an article recently published in Blood, Elliott et al 1 reported that commercially available anti–erythropoietin receptor (anti-EpoR) antibodies are hardly usable to predict EpoR expression because of their low specificity and affinity. Although we agree with the authors that many of these antibodies work poorly, we strongly disagree with them concerning 2 main points: (1) the ability of San...

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Anti-Epo receptor antibodies do not predict Epo receptor expression.

Investigators using anti-EpoR antibodies for immunoblotting and immunostaining have reported erythropoietin receptor (EpoR) expression in nonhematopoietic tissues including human tumors. However, these antibodies detected proteins of 66 to 78 kDa, significantly larger than the predicted molecular weight of EpoR (56-57 kDa). We investigated the specificity of these antibodies and showed that the...

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ژورنال

عنوان ژورنال: Nature Biotechnology

سال: 2000

ISSN: 1087-0156,1546-1696

DOI: 10.1038/74354